Tube staff have voted for industrial action, including strikes, over plans to move the Piccadilly Line Service Control centre to Hammersmith Service Control Centre.
The affected staff are members of the RMT union which says London Underground has failed to recognise a number of concerns around pay and conditions.
The union says 70% of those who voted backed strike action while 80% voted for action short of a strike.
RMT General Secretary Bob Crow said: “This group of workers are absolutely key to delivering the service on the Piccadilly line and sub-surface railway and the way that they have been treated by the management at a time when the system is under intense pressure is nothing short of shocking.
“These are the same key staff who keep services running when demand is at a peak and they are the same staff who manage the problems when the lines are hit by breakdowns and failures. They deserve respect for the job that they do and not the imposition of inferior working conditions and the threat to jobs.”
The union says it “remains available for meaningful talks.”