The 36 mile PRN will be used used to get athletes, Games officials and media to events on time.
The Network includes 8.7 miles of Games lanes reserved for Games traffic.
The PRN and the Games lanes will be in operation between 06:00 to 00:00 daily Wednesday 29 August until Monday 10 September, the day after the closing ceremony of the Paralympic Games.
Transport for London says the network and lanes will be “enforced flexibly”.
London’s Transport Commissioner, Peter Hendy said: “While the Paralympics are smaller than the Olympics, they will present their own set of challenges. London’s children will begin a new school year in the second week – and their parents will return to work – and larger groups are expected to travel together on the public transport network.
“The ORN played a vital role in the success of the Olympics, getting all athletes, officials and the world’s media to their Games events on time and to keep London moving, and now we need the PRN to do the same job. Our advice to motorists is to avoid driving around the PRN routes and venues, particularly the Olympic Park in Stratford and on the A102 approach roads north and south of the Blackwall Tunnel.
“Please continue to plan ahead at GetAheadoftheGames.com and allow extra time for your journeys when necessary.”
